What color is Alexa Fluor 594?
red-fluorescent
Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines.
What color is 594 nm?
Table 1. Properties of Alexa Fluor Dyes
| Alexa Fluor dye | Absorption max. (nm) | Emission color* |
|---|---|---|
| Alexa Fluor 546 | 556 | Orange |
| Alexa Fluor 555 | 555 | Orange |
| Alexa Fluor 568 | 578 | Orange/Red |
| Alexa Fluor 594 | 590 | Red |
What does Alexa Fluor 488?
For example, Alexa Fluor 488, a sulfonated and otherwise chemically modified form of fluorescein, was designed to solve the well known issues of rapid photobleaching and pH-dependent fluorescent intensity characteristic of the dye fluorescein isothiocyanate.
What does Alexa Fluor 594?
Invitrogen Alexa Fluor 594 dye is a bright, red-fluorescent dye that can be excited using the 561 nm or 594 nm laser lines. We offer Alexa Fluor 594 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection.
What wavelength is DAPI?
358 nm
When bound to double-stranded DNA, DAPI has an absorption maximum at a wavelength of 358 nm (ultraviolet) and its emission maximum is at 461 nm (blue). Therefore, for fluorescence microscopy, DAPI is excited with ultraviolet light and is detected through a blue/cyan filter.
Which has a higher frequency red or Green?
The visible spectrum
| colour* | wavelength (nm) | frequency (1014 Hz) |
|---|---|---|
| red | 650 | 4.62 |
| orange | 600 | 5.00 |
| yellow | 580 | 5.16 |
| green | 550 | 5.45 |
Is Cy3 red or green?
Cy3 fluoresces greenish yellow (~550 nm excitation, ~570 nm emission), while Cy5 is fluorescent in the far-red region (~650 excitation, 670 nm emission). Cy3 can be detected by various fluorometers, imagers, and microscopes with standard filters for Tetramethylrhodamine (TRITC).
Is Alexa Fluor 488 Green?
Invitrogen Alexa Fluor 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. …
How long do Alexa Fluor antibodies last?
Storage at 4°C should not exceed 1 or 2 weeks.
What Alexa 647?
Alexa Fluor® 647 is a bright and photostable far-red dye with excitation ideally suited to the 633 nm laser line. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor® 647 dye is water soluble and pH-insensitive from pH 4 to pH 10.
What wavelength is Tritc?
532 nm
Tetramethylrhodamine (TRITC) is a bright orange-fluorescent dye with excitation ideally suited to the 532 nm laser line. It is commonly conjugated to antibodies and proteins for cellular imaging applications.