What is Eppendorf PCR machine?
The flexible, versatile Eppendorf Mastercycler® nexus PCR cycler. All (7) Images (7) All (7) The versatile Mastercycler® nexus enables you to select the PCR consumables that best fit your assay. The nexus PCR cycler can accommodate 96-well PCR plates, 0.2 mL PCR tubes, 0.2 mL PCR tube strips, and 0.5 mL PCR tubes.
How much does a PCR thermocycler cost?
A simple PCR machine like Bio-Rad T100 thermal cycler has a list price of 4912 USD (with a promotional price of 2595 USD in the US) as of Jan 30, 2019. The cost of rtPCR systems ranges anywhere from 15,000$ for some RotorGene models to over 90,000$ for QuantStudio 12k.
How many different mastercycler thermal cycler variants does Eppendorf offer?
The Eppendorf Thermal Sample Protection (TSP) prevents premature heating of your samples during the heating phase of the heated lid, keeping nonspecific PCR products from amplification during this phase and preserving your samples. 3 variations of this product are available.
What is a PCR device?
Polymerase chain reaction (PCR) machines are cost-effective and highly efficient tools used to amplify small segments of DNA or RNA. The technique works by binding primers to the target sequence and extending this using a Taq polymerase.
What is master cycler?
The versatile Mastercycler® nexus enables you to select the PCR consumables that best fit your assay. The nexus PCR cycler can accommodate 96-well PCR plates, 0.2 mL PCR tubes, 0.2 mL PCR tube strips, and 0.5 mL PCR tubes.
How does a thermal cycler help with the process of PCR?
Thermocyclers, or thermal cyclers, are instruments used to amplify DNA and RNA samples by the polymerase chain reaction. The thermocycler raises and lowers the temperature of the samples in a holding block in discrete, pre-programmed steps, allowing for denaturation and reannealing of samples with various reagents.
What are the 6 steps of PCR?
The following is a typical PCR thermocycler profile.
- Initialization. In this step, the reaction is heated to 94–96°C for 30 seconds to several minutes.
- Denaturation (Repeated 15–40 Times)
- Annealing (Repeated 15–40 Times)
- Elongation or Extension (Repeated 15–40 Times)
- And Repeat…
- Final Elongation.
- Final Hold.
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What is gradient PCR?
Gradient PCR is a technique that allows the empirical determination of an optimal annealing temperature using the least number of steps. The Eppendorf Mastercycler Gradient provides a gradient function that in one single run evaluates up to 12 different annealing, elongation, or denaturation temperatures.
What is PCR and its application?
Polymerase chain reaction, or PCR, is a technique to make many copies of a specific DNA region in vitro (in a test tube rather than an organism). PCR has many research and practical applications. It is routinely used in DNA cloning, medical diagnostics, and forensic analysis of DNA.
When did Eppendorf Start Making PCR cyclers?
In 2003, we launched the PhysioCare Concept ®, focusing on ergonomic liquid handling devices, like our pipettes. Since then we’ve broadened the Eppendorf PhysioCare concept to include many laboratory products, including PCR cyclers.
Why do we use Eppendorf fast PCR tube strip?
Allow us to introduce the custom engineered vessel: The Eppendorf Fast PCR Tube Strip. The Fast PCR Tube Strip allows faster transfer of heat from block to reaction, allowing significantly better control over the reaction parameters. The results: For more info, see application note 400.
Can a PCR cycler be used in a laboratory?
Since then we’ve broadened the Eppendorf PhysioCare concept to include many laboratory products, including PCR cyclers. Our aim is to offer a holistic solution for harmonizing the workflow in your laboratory with your health and wellbeing.
Is the Eppendorf mastercycler Nexus in standby mode?
Eppendorf’s Mastercycler nexus is optimized for low power consumption – regardless of whether the cycler is running, idle, or in standby mode. Suffering from noise in the lab? With many different instruments in the lab, noise levels can easily become too high. The Mastercycler nexus is a whisper quiet PCR cycler.