What is Prussian blue stain used for?
Iron Stain Kit (Prussian Blue stain) ab150674 is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen. Abnormally large deposits may be seen in hemochromatosis and hemosiderosis.
What is the Prussian blue stain used for in relation to a bone marrow aspiration?
The stain is an important histochemical stain used to demonstrate the distribution and amount of iron deposits in liver tissue, often in the form of a biopsy. Perls Prussian blue is commonly used on bone marrow aspirates to indicate levels of iron storage and may provide reliable evidence of iron deficiency.
How does Prussian blue stain work?
Prussian blue reaction involves the treatment of sections with acid solutions of ferrocyanides. Any ferric ion (+3) present in the tissue combines with the ferrocyanide and results in the formation of a bright blue pigment called Prussian blue, or ferric ferrocyanide.
What pigment does Prussian blue stain?
Hemosiderin is an iron-containing, golden-brown, granular pigment derived from ferritin, the initial iron-storage protein. As iron accumulates within the cell, aggregates of ferritin molecules form hemosiderin (E-Fig. 8-6).
Is Prussian blue a poison?
Despite the fact that it is prepared from cyanide salts, Prussian blue is not toxic because the cyanide groups are tightly bound to iron.
What is Prussian blue reaction?
Prussian Blue or Perls’ reaction is used to demonstrate ferric iron and ferritin. The protein is split off by the hydrochloric acid, allowing the potassium ferrocyanide to combine with the ferric iron. This forms the ferric ferrocyanide or Prussian Blue.
What does Luxol fast blue stain?
Luxol fadt blue stain (LFB) is used to visualize myelin in nerve tissue. Thanks to this staining, it is possible to visualize the myelin and the fatty substance of the brain called white matter which includes the axons of the nerve cells.
Does Prussian blue stain lipofuscin pigment?
Lipofuscin is also a yellow-brown, finely granular pigment; however, it is derived mainly from the breakdown products of lipids, such as cell membranes. Hemosiderin can be identified with iron stains such as Perl’s iron and Prussian blue, both of which stain the pigment blue.
What does Prussian blue symbolize?
Prussian blue is a common histopathology stain used by pathologists to detect the presence of iron in biopsy specimens, such as in bone marrow samples.
Which is Prussian blue stain for bone marrow smear?
Product overview Iron Stain Kit (Prussian Blue stain) ab150674 is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen. Abnormally large deposits may be seen in hemochromatosis and hemosiderosis.
What do you use Prussian blue stain for?
Product overview Iron Stain Kit (Prussian Blue stain) ab150674 is intended for use in the detection of ferric iron in tissues, blood smears, or bone marrow smears. Ferric iron is normally found in small amounts in bone marrow and the spleen.
Why do you use Perls Prussian blue on bone marrow?
Perls Prussian blue is commonly used on bone marrow aspirates to indicate levels of iron storage and may provide reliable evidence of iron deficiency. Perls did not publish a detailed procedure other than indicating a dilute potassium ferrocyanide solution was applied to the tissue followed by hydrochloric acid.
What is Prussian blue used for in hematology?
Prussian blue (Perls’) reaction is a method for staining non-haem iron in normoblasts (siderocytes), macrophages (haemosiderin), and other cells containing particulate iron.