What is Sequenom MassARRAY?
iPLEX® Gold is a leading technology for SNP Genotyping. The MassARRAY system is widely used for fine mapping and validation of GWAS studies linkage studies, as well as routine genetic testing of SNP panels of interest.
How does MassARRAY work?
The MassARRAY System is a Matrix-Assisted Laser Desorption Ionization – Time of Flight (MALDI-TOF) mass spectrometer customized for the precise detection of DNA molecules. The molecules are distinguished by their time-of-flight after being ionized in a vacuum chamber.
What is SpectroChip?
Our technology integrates spectral sensor, computation algorithm, Apps and cloud. SpectroChip can be embedded in handheld devices or in smart phones to perform spectral sensing of test strips and reagents, which can reveal the personal biological data as well as all sorts of test items.
How do you measure SNP?
SNP genotyping
- SNP genotyping is the measurement of genetic variations of single nucleotide polymorphisms (SNPs) between members of a species.
- In the first step, a genomic segment is amplified and attached to a bead through a PCR reaction with a biotinylated primer.
What is Maldi TOF mass spectrometry?
MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry is a new technology that has revolutionized pathogen identification and has also proven to accelerate detection of antimicrobial resistance compared to the traditional antibiotic susceptibility tests (AST) as well as DNA …
What causes a SNP?
On the most basic level SNPs are simply a product of chemical reactions leading to base substitutions/removal in DNA particles. DNA repair systems developed in living cells as an answer to this damage but they are not error-free.
What is SNP mapping?
Abstract. Single-nucleotide polymorphism (SNP) mapping is the easiest and most reliable way to map genes in Caenorhabditis elegans. SNPs are extremely dense and usually have no associated phenotype, making them ideal markers for mapping. SNP mapping has three steps.
What is an example of SNP?
An example of an SNP is the substitution of a C for a G in the nucleotide sequence AACGAT, thereby producing the sequence AACCAT. The DNA of humans may contain many SNPs, since these variations occur at a rate of one in every 100–300 nucleotides in the human genome.
What is the difference between a SNP and a mutation?
Mutation is any kind of variation in the genome, including addition, deletion, duplication, substitution and… .But SNPs are just single-nucleotide substitutions of one base for another that occur in more than one percent of the general population. And frequency of mutation is less than one percent.
What are the four stages of a mass spectrometry?
There are four stages in a mass spectrometer which we need to consider, these are – ionisation, acceleration, deflection, and detection.