How does T7 promoter work?
T7 RNA polymerase is a very active enzyme: it synthesizes RNA at a rate several times that of E. coli RNA polymerase and it terminates transcription less frequently; in fact, its transcription can circumnavigate a plasmid, resulting in RNA several times the plasmid length in size.
What is T7 RNA polymerase used for?
T7 RNA Polymerase is used for in vitro mRNA synthesis and is highly specific for the T7 phage promoter. Applications include: Radiolabeled RNA probe preparation. RNA generation for studies of RNA structure, processing and catalysis.
How is T7 transmitted?
Infection of host bacteria T7 is using its fibers to “walk” across the cell surface and finally infect the cell.
What is the T7 expression system?
The T7 expression system allows high-level expression from the strong bacteriophage T7 promoter. It’s ideal for expressing soluble, nontoxic recombinant proteins in E. coli. The T7 expression vectors are designed to facilitate cloning using Gateway® technology, and easy protein purification and detection.
Where does T7 promoter come from?
T7 RNAP is a small (99 kDa) DNA-dependent RNAP derived from bacteriophage T7 (Chamberlin and Ring, 1973; Golomb and Chamberlin, 1974; Steitz, 2004). The polymerase efficiently and specifically transcribes genes bearing a T7 promoter (PT7).
Why is T7 important?
Significantly, the T7 enzyme catalyzes RNA synthesis 5–10 times faster than the host RNAP. As mentioned, besides ΦM and ΦL, there are several other promoters distributed among class II and III genes that are transcribed by T7 RNAP, and transcription from these promoters increases the products of certain genes.
Where is the T7 virus found?
Hosts. T7 grows on rough strains of Escherichia coli (i.e. those without full-length O-antigen polysaccharide on their surface) and some other enteric bacteria, but close relatives also infect smooth and even capsulated strains. E. coli is more resistant to T7 than to some other similar phages.
Does the T7 virus have a vaccine?
T7 phage may be potentially useful as a delivery vector for DNA vaccine transfer. The surface display capability of T7 phage also enlarge the use in vaccine design, for it can surface display antigen epitope and carry DNA vaccine within one particles.
Where does T7 RNA polymerase come from?
T7 RNA Polymerase is an RNA polymerase from the T7 bacteriophage that catalyzes the formation of RNA from DNA in the 5’→ 3′ direction.
Where can I buy Promega T7 RNA polymerase?
This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab’s needs. T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences.
Which is a template for T7 RNA polymerase?
We offer numerous convenient solutions to meet your lab’s needs. T7 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only T7 DNA or DNA cloned downstream from a T7 promoter can serve as a template for T7 RNA Polymerase-directed RNA synthesis.
Where can I get a Promega p1700 Technical Bulletin?
P1700 TECHNICAL BULLETIN Promega Corporation · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax 608-277-2516 www.promega.comTB316 · Revised 12/13