Is Mycobacterium tuberculosis nitrate positive?

tuberculosis and Mycobacterium canettii strains gave a strongly positive nitrate reductase result within 1day of positive detection by the MGIT960 system.

What technique could be used to identify Mycobacterium tuberculosis?

PCR amplification of mycobacterial DNA is a highly sensitive and specific technique to detect Tuberculosis. This also performs better than smear (which needs >10,000 bacilli/mL sample for detection) as well as culture method (which has restricted growth conditions).

Which test is done for susceptibility to tuberculosis?

The LB uses an indirect proportion method for testing M. tuberculosis complex mycobacteria to 12 drugs at 35C on Middlebrook 7H10 agar. The test requires 1 month to complete. Growth on the control medium is compared to the growth on the drug-containing medium to determine susceptibility or resistance.

What is the genetic basis of drug resistance in Mycobacterium tuberculosis?

The resistance is provoked by mutations in the rpsL gene, which encoded the S12 protein, and the rrs gene, which encoded the 16S rRNA. The mutations in both genes are the main mechanism of streptomycin resistance, and it has been found in 65–67% of resistant clinical isolates [16].

How do you perform a nitrate reduction test?

The nitrate reduction test tests for a bacterial strain’s ability to reduce nitrate to nitrite or further compounds. To perform the test, first inoculate a tube of nitrate broth with a pure culture of your test organism and incubate it at 37 degrees Celsius for 5-10 days.

Which method is used to identify Mycobacterium?

PCR methods
PCR methods allow direct identification of Mycobacterium tuberculosis complex and can detects less than 10 bacteria in a clinical specimen. PCR sensitivity ranges from 70-90% compared to the result of culture and its specifty varies between 90 and 95%.

How can you prevent Mycobacterium tuberculosis?

The risk of infection can be reduced by using a few simple precautions:

  1. good ventilation: as TB can remain suspended in the air for several hours with no ventilation.
  2. natural light: UV light kills off TB bacteria.
  3. good hygiene: covering the mouth and nose when coughing or sneezing reduces the spread of TB bacteria.

What should be done when a health care worker suspects that a patient has TB?

Persons who have or are suspected of having infectious TB disease should be placed in an area away from other patients, preferably in an airborne infection isolation (AII) room.

What does it mean if a bacteria is sensitive to an antibiotic?

Susceptible means they can’t grow if the drug is present. This means the antibiotic is effective against the bacteria. Resistant means the bacteria can grow even if the drug is present. This is a sign of an ineffective antibiotic. Intermediate means a higher dose of the antibiotic is needed to prevent growth.

What drugs are Mycobacterium tuberculosis resistant to?

TB experts should be consulted in the treatment of MDR TB. Extensively drug-resistant TB (XDR TB) is a rare type of MDR TB that is resistant to isoniazid and rifampin, plus any fluoroquinolone and at least one of three injectable second-line drugs (i.e., amikacin, kanamycin, or capreomycin).

Why does TB become drug resistant?

Drug resistance in TB remains a man-made phenomenon. It emerges as a result of spontaneous gene mutations in M. tuberculosis that render the bacteria resistant to the most commonly used anti-TB drugs. Among the reasons for this, the non-compliance with the treatment regimens is signaled as the first cause.

What are the 2 major end product of nitrate reduction?

With this enzyme, nitrate is reduced to nitrite (NO2). It then forms nitrous acid that reacts with the first reagent sulfanilic acid, and that reacts with the other reagent naphthylamine to form a red color. Reduction of nitrate is generally an anaerobic respiration in which an organism derives its oxygen from nitrate.