What does Western blot results interpreted as indeterminate?
A person whose Western blot test results continue to be consistently indeterminate for at least 6 months–in the absence of any known risk factors, clinical symptoms, or other findings–may be considered to be negative for antibodies to HIV-1.
When do Western blot results interpret indeterminate?
The result is considered indeterminate when one or more bands are present but do not meet the criteria for a positive Western blot result. A positive HIV-1 Western blot result following a positive EIA result for HIV-1 or HIV-2 is diagnostic of established HIV-1 infection.
How do you read a Western blot test?
The Western blot assay detects antibodies that react with specific proteins (antigens) of the HIV-1 virus….Human Immunodeficiency Virus Western Blot.
| Interpretation | Bands Present |
|---|---|
| Positive | Presence of any 2 bands; p24, gp41, gp120/160 |
| Negative | absence of bands |
| Indeterminate | any other combination of bands |
What diseases can Western blot detect?
In recent medical field, Western Blot has a wide range of applications in medical diagnosis, such as the application of medical diagnosis for HIV (Human Immunodeficiency Virus) infection, BSE (Bovine Spongiform Encephalopathy, also known as “mad cow disease”), FIV (Feline Immunodeficiency Virus), HBV (Hepatitis B Virus …
Is Elisa and Western blot the same?
Both ELISA and Western Blotting are indirect tests for use in analyzing a wide variety of samples. ELISA is a simpler and faster procedure than Western blotting, which is less specific. Western Blotting is a highly successful testing method for confirming positive results from ELISA tests.
When would you use ELISA vs Western blot?
ELISA is a simpler and faster procedure than Western blotting, which is less specific. Western Blotting is a highly successful testing method for confirming positive results from ELISA tests. It is also used as a confirmatory test as it is difficult to perform and requires a high skill level.
What causes indeterminate results on a western blot?
Indeterminate Western Blot tests account for 4% to 20% of Western Blot assays in various studies. The causes of indeterminate results include: Serologic tests in the process of seroconversion; anti-p24 is usually the first antibody band to appear. Late-stage HIV infection, usually with loss of core antibody.
What are the interpretive standards for Western blot?
The interpretive standards that require the identification of bands from each of the three groups of gene products tend to have indeterminate results for some AIDS and other symptomatic patients due to absence of antibodies to p24 (n=5) or to p31 (n=14) or absence of both types of antibodies (n=2).
What makes a negative du Pont western blot test negative?
Conversely, a negative Du Pont Western blot test result requires the absence of any and all bands–not just viral-bands. All other patterns are regarded as indeterminate.
When to run an indeterminate western blot for HIV?
If a Western Blot turns up two or more reactive bands, the patient is considered HIV-positive. If it turns up one band or several “equivocal” bands, the test is considered “indeterminate” and should be run again in about a month. That monthlong wait speaks to perhaps the most insidious aspect of the AIDS virus: It hides.