What happens when beta catenin is phosphorylated?
β-Catenin is phosphorylated on its N-terminus by GSK3 (shown in orange) and CKI (shown in orange). These phosphorylations target β-catenin for ubiquitination and subsequent proteasomal degradation.
What is active beta catenin?
Catenin beta-1, also known as β-catenin, is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a dual function protein, involved in regulation and coordination of cell–cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene.
Is beta catenin a coactivator?
Previous studies have shown that β-catenin activates TCF-mediated transcription in various cell lines (4). 2B), further supporting the role of p300 as a coactivator for β-catenin/TCF-mediated transcription.
How do you lower catenin beta?
The modulators of β-catenin degradation Efforts are made to activate kinase activity and thus to reduce the stability of β-catenin. Axin is an important protein in promoting β-catenin degradation. Tankyrase1/2 is a kinase destabilizing Axin, which is chosen as a target to reduce the accumulation of β-catenin.
What genes does beta-catenin activate?
In the nucleus β-catenin activates transcription of Wnt/β-catenin-target genes such as c-myc and cyclinD1 (Maretzky et al, 2005; Reiss et al, 2005).
Is beta-catenin a gene?
The CTNNB1 gene provides instructions for making a protein called beta-catenin. This protein is present in many types of cells and tissues, where it is primarily found at junctions that connect neighboring cells (adherens junctions).
Can a non phospho Mab detect β-catenin?
Note that Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb fails to detect poly-ubiquitinated β-catenin in MG132-treated cells, indicating its specificity for stabilized protein. Immunohistochemical analysis of paraffin embedded human breast carcinoma using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb.
Is there absence of signal in β catenin knockout HeLa cells?
The absence of signal in the β-Catenin knockout HeLa cells confirms the specificity of the antibody for β-Catenin. Western blot analysis of extracts from 293T cells, untreated (-) or treated (+) with LiCl (20 mM, 20 hr at 37ºC), using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb (left) and β-Catenin Antibody #9562 (right).
Where does TCF4 and β-catenin bind?
TCF4 and β-Catenin are known to associate with each other on chromatin. The figure shows binding of both TCF4 and β-Catenin across CAMK2D, a known target gene of both TCF4 and β-Catenin (see additional figure containing ChIP-qPCR data).