What is fragment Analyzer?

The Fragment Analyzer ™ system is a parallel capillary electrophoresis instrument, which performs DNA QC and RNA QC for a broad range of samples including, gDNA, small RNA, cfDNA, large DNA fragments, and total RNA.

What is a fragment Analyzer used for?

The Fragment Analyzer Auto Capillary Electrophoresis system from Advanced Analytical Technologies Inc is used for accurately sizing and quantifying DNA or RNA.

How much does a fragment analyzer cost?

Price

Service on the Fragment Analyzer UGA Non-UGA
High sensitivity Large Fragment analysis (per sample) $7.80 $9.25
High sensitivity Large Fragment analysis (per run*) $80.50 $95.00
High sensitivity Large Fragment analysis (half plate **) $255.00 $301.00
High sensitivity Large Fragment analysis (96-well plate ***) $505.00 $596.00

How fragment Analyzer works?

The Fragment Analyzer™ system performs parallel separations simultaneously via multiplexed capillary electrophoresis. By using a sensitive intercalating dye coupled with a powerful LED light source, sizing and quantification of both DNA fragments and RNA samples are performed.

What is a TapeStation?

TapeStation Systems The Agilent TapeStation system is an automated electrophoresis solution for the sample quality control of DNA and RNA samples. The system integrates an instrument, data processing software, reagents, and ScreenTape devices specific for DNA and RNA.

What is the difference between Bioanalyzer and TapeStation?

A: The Agilent 2200 TapeStation system can run anywhere from 1 to 95 samples. The Bioanalyzer is “chip- based” and can only fit 11 or 12 samples per run. Therefore, the service fee for Bioanalyzer run is charged on a per chip basis. Both Bioanalyzer and TapeStation can run cDNA or NextGen library samples.

What does the word fragment?

noun. a part broken off or detached: scattered fragments of the broken vase. an isolated, unfinished, or incomplete part: She played a fragment of her latest composition. an odd piece, bit, or scrap.

What is a Bioanalyzer?

The Bioanalyzer is a chip-based capillary electrophoresis machine to analyse RNA, DNA, and protein. It is produced by Agilent and widely used, among other things, in RNA quality control measurements before downstream experiments like microarrays.

How does a DNA analyzer work?

Genetic analyzers are automated systems capable of sequencing DNA or analyzing fragments for a variety of applications. In capillary electrophoresis-based systems, DNA fragments bound to probes migrate through a polymer and the fluorescence emissions are measured.

How does Agilent TapeStation work?

The Agilent TapeStation system is an automated electrophoresis solution for the sample quality control of DNA and RNA samples. The system integrates an instrument, data processing software, reagents, and ScreenTape devices specific for DNA and RNA.

How does the Agilent Bioanalyzer work?

The Agilent 2100 Bioanalyzer is a microfluidics-based platform for sizing, quantification and quality control of DNA and RNA. The fluorescent dye molecules intercalate into DNA or RNA strands. They are then detected by their fluorescence and translated into gel-like images (bands) and electropherograms (peaks).

What does the 5300 fragment analyzer system do?

This system is designed to improve lab efficiency and workflow allowing you to automate the analysis of large numbers of samples and focus on results. The 5300 Fragment Analyzer system performs DNA QC and RNA QC for a broad range of samples including, gDNA, small RNA, cfDNA, large DNA fragments, and total RNA.

How does fragment analysis work in Thermo Fisher?

The resulting peaks are then processed according to the user-specified analysis parameters and size standard definition in the primary and secondary analysis software. These tools convert the images gathered during Data Collection into sized fragment length peaks (Figure 2).

How are amplified fragments detected in DCS software?

Amplified fragments, along with appropriate size standards, migrate through a polymer-filled capillary and are detected using Data Collection Software (DCS).